Molecular cloning and functional analysis of flavanone 3-hydroxylase (f3h）gene in Passiflora edulis Sims

Flavanone 3-hydroxylase (F3H) plays a crucial role in the biosynthesis of flavonoids. In present study, one F3H gene (P_edulia040010337.g) from Passiflora edulis Sims, which has coding sequence (CDS) 1161 bp, encoding protein consisting 386 amino acid residues was cloned. The PeF3H contains non-heme dioxygenase (DIOX-N superfamily) domain and typical functional (2OG-FeII-Oxy dioxygenase). Phylogenetic analysis revealed that shared high similarity with proteins Turnera subulata, Populus alba, tomentosa, 88% identities sequences. lacks transmembrane structure, indicating it is likely to be expressed mitochondria. Additionally, 3D interaction, KEGG pathway were anticipated based on homologous proteins. qRT-PCR showed highly leaves, followed by stems roots. These studies have provided insights into molecular mechanisms underlying flavonoid predicted potential targets for genetic engineering improve nutritional medicinal properties passion fruit. Bangladesh J. Bot. 52(2): 613-623, 2023 (June) Special